Shortened PCR Cycles in a Conventional Thermal Cycler
نویسندگان
چکیده
منابع مشابه
Shortened PCR cycles in a conventional thermal cycler.
step. The presence of Vent DNA polymerase and PCR products other than the intended DNA linker, however, did not seem to interfere with subsequent reactions because ligations using both gel-purified and unpurified PCR products yielded a similar number of transformants. Eight transformants were tested, all of which contained the PCR product as an insert. The yield was considered to have high effi...
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This paper presents a PC-based thermal cycler with a local-host architecture, where a PC is employed as the host. The local system only executes the thermal cycling process and the PC is responsible for the management of PCR protocols with a convenient GUI. The presented local-host system greatly reduces the development time and the maintenance cost, providing an economical PCR thermal cycler.
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1998
ISSN: 0736-6205,1940-9818
DOI: 10.2144/98252bm06